On a whole-animal method for the histochemical localization of enzymes under field conditions
Southward, A.J. and Southward, E.C. (1968) On a whole-animal method for the histochemical localization of enzymes under field conditions. Journal of the Marine Biological Association of the United Kingdom, 48 (2). pp. 323-334.
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Various enzymes have been successfully localized in whole specimens of several groups of marine animals up to 5 mm body thickness. With certain exceptions the animals are fixed for a short time and then incubated in media modified from those designed for sections. After incubation the specimens are stored in fixative or buffer for later sectioning back at the laboratory. Some general aspects of the technique are discussed, and details given of modified media for esterases, phosphatases, peptidases and dehydrogenases. The use of whole animals allows investigation of deep-sea and other oceanic animals that cannot be studied in the laboratory, and the technique may be generally useful for work under rough conditions.
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