Observations on the keeping of adult female Pseudocalanus elongatus under laboratory conditions
Corkett, C.J. and Urry, D.L. (1968) Observations on the keeping of adult female Pseudocalanus elongatus under laboratory conditions. Journal of the Marine Biological Association of the United Kingdom, 48 (1). pp. 97-105.
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Stocks of adult female Pseudocalanus elongatus have been kept for the past 6 years in a laboratory remote from the sea. The techniques used in maintaining these stocks are described. The most suitable temperature for keeping the copepods has been found to be l0 degrees centigrade. The control of pH has not been necessary. Although an allowance of 30 ml. of sea water for each copepod is recommended, it has been found that survival is not affected by densities of up to I copepod/ 10 ml. It has been shown that Erdschreiber medium does not provide nutrition for P. elongatus. The two most satisfactory grazing media for laboratory stocks of P. elongatus are Isochrysis galbana and Lauderia borealis. Penicillin and streptomycin can be used in sufficient concentration to check bacterial contamination without deleterious effects either on Pseudocalanus elongatus or Isochrysis galbana.
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